Expression and mechanism of miR - 429 in patient with bladder cancer
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摘要:
目的 探讨miR-429在膀胱癌中的表达及作用机制。 方法 选取广东省内三家医院2014年1月~2016年12月期间收集的膀胱癌标本120例，同时选取距离肿瘤边缘大于2 cm的癌旁组织作为对照。SYBR green实时荧光定量PCR法检测miR-429在膀胱癌、癌旁组织中的相对表达量，并分析miR-429与临床相关参数的关系，同时观察miR-429过表达或者敲除时T24细胞（膀胱癌细胞系）增殖和凋亡情况。 结果 膀胱癌组织miR-429的相对表达水平高于正常膀胱组织（P<0.05）；miR-429的表达水平与膀胱癌的肿瘤大小、有无淋巴结转移，及TNM分期有关（P<0.05）；肿瘤直径大于5 cm组miR-429的表达水平高于肿瘤直径小于5 cm组；有淋巴结转移的膀胱癌组miR-429的表达水平高于无淋巴结转移的膀胱癌组；Ⅲ+Ⅳ期膀胱癌组miR-429的表达水平高于Ⅰ+Ⅱ期膀胱癌（P<0.05）。miR-429的表达水平与膀胱癌患者的年龄、性别无关（P>0.05）。miR-429过表达时，膀胱癌细胞（T24细胞系）增殖增加，凋亡减少；而miR-429敲除时，膀胱癌细胞（T24细胞系）增殖减少，凋亡增加。 结论 miR-429在膀胱癌的发生发展中起着重要作用，其表达水平对膀胱癌的严重程度及预后情况具有一定的参考价值。 Abstract:Objective To explore the expression and mechanism of miR-429 in bladder cancer. Methods A total of 120 cases with bladder cancer were collected from our hospital in 2015.1-2016.12. The adjacent tissues with the edge >2 cm were selected as the control. All specimens were confirmed by pathological diagnosis without preoperative radiotherapy and chemotherapy. The relative expression of miR-429 in bladder cancer and adjacent tissues were analyzed by SYBR green real-time quantitative PCR. The relative expression of miR-429 in bladder cancer carcinoma was explored and the relationship between miR-429 and clinical features was analyzed. Results The relative expression level of miR-429 in bladder cancer tissues was significantly higher than that in normal bladder tissues (P<0.05).The expression level of miR-429 was associated with tumor size, lymph node metastasis and TNM staging of bladder cancer (P<0.05). The expression level of miR-429 in tumor tissue with diameter greater than 5 cm was higher than that in tumor group with diameter less than 5 cm. The expression level of miR-429 in bladder transition group was higher than that in bladder transition group without lymph node metastasis.The expression level of miR-429 was significantly higher than that of stage Ⅰ + Ⅱ bladder cancer (P<0.05). The expression level of miR-429 was not related to the age and sex of bladder cancer patients (P>0.05). miR-429 overexpression, bladder cancer cells (T24 cell line) proliferation increased and apoptosis reduced.miR-429 knockout, bladder cancer cells (T24 cell line) proliferation reduced.The apoptosis increased. Conclusion miR-429 is important in the development and progression of bladder cancer. Expression of miR-429 can provide references for the severity and prognosis of bladder cancer. -
Key words:
- bladder cancer /
- miR-429 /
- microRNA
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图 2 miR-429过表达或者敲除时T24细胞增殖和凋亡情况
A, B: miR-429过表达或者敲除时T24凋亡情况比较; C, D: miR-429过表达或者敲除时T24增殖情况比较; P<0.05 vs 对照组.
表 1 膀胱癌一般情况分布表（n=120）
变量 例数（%） 年龄（岁） <60 40（33.3%） ≧60 80（66.7%） 性别 男 50（41.7%） 女 70（58.3%） 肿瘤大小（cm） ≧5 16（13.3%） <5 104（86.7%） 有无淋巴结转移 有 60（50%） 无 60（50%） TNM分期 Ⅰ+Ⅱ期 44（36.7%） Ⅲ+Ⅳ期 76（63.3%） 
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表 2 miR-429的表达水平与膀胱癌临床相关参数的关系
变量 病例数 miR-429相对表达量 P 年龄（岁） <60 40 1.127 >0.05 ≧60 80 1.255 性别 男 50 1.154 >0.05 女 70 1.141 肿瘤大小（cm） ≧5 16 1.397 <0.05 <5 104 1.001 有无淋巴结转移 有 60 1.423 <0.05 无 60 1.021 TNM分期 Ⅰ+Ⅱ期 82 0.861 <0.05 Ⅲ+Ⅳ期 38 1.685 Two-sided Fisher’s精确检验.
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