Effects of PRDM1gene expression on the reproductive ability of non-Hodgkin lymphoma cells
-
摘要:
目的 观察PRDM1对非霍奇金淋巴瘤细胞SUDHL-4增殖能力的影响。 方法 通过质粒转染的方法在低表达PRDM1的SUDHL-4细胞中过表达PRDM1基因，通过MTT实验、细胞周期分析检测SUDHL-4生物学行为的变化。 结果 过表达PRDM1的SUDHL-4细胞的生长速度明显下降，细胞G0/G1比例、S期比例下降；G2/M期比例升高,细胞阻滞于G2/M期，差异均有统计学意义（P<0.05）。 结论 PRDM1基因表达缺失可能在非霍奇金淋巴瘤的发生发展中起重要作用，可能成为判断非霍奇金淋巴瘤预后的分子标志物及临床治疗的靶点。 Abstract:Objective To explore the effects of PRDM1gene expression on the reproductive ability of Non-Hodgkin Lymphoma cells SUDHL-4． Methods Non-Hodgkin Lymphoma cells SUDHL-4 was chosed to show the biology behavior changes after overexpressing PRDM1 gene, through the MTT test, and cell cycle analysis. Results After overexpressing PRDM1 gene, the proliferation of SUDHL-4 cell were significantly decreased, and cell cycle arrested at G2/M (P<0.05). Conclusion The loss of PRDM1 gene may be related to the occurrence and development of lymphoma, and it may become the molecular diagnostics and new therapy target of lymphoma. -
Key words:
- non-Hodgkin lymphoma /
- PRDM1 /
- SUDHL-4 cell
-
图 1 在SUDHL-4 中转染pcDNA3.1(+)-PRDM1后，PRDM1表达量增加
A:SUDHL-4 中转染pcDNA3.1(＋)-PRDM1、pcDNA3.1(+)及空白对照后，PRDM1表达情况; B: PRDM1与内参β-actin相对表达量.
表 1 细胞周期分析结果（%）
组别 G0/G1 S G2/M SUDHL-4-PRDM1 40.1±3.2 * 38.5±3.7* 26.1±2.9 * SUDHL-4-vector 48.9±3.6 48.7±3.3 10.1±1.6 SUDHL-4 47.2±3.1 49.6±3.5 9.2±1.8 *P ＜0.05 vs 对照组. 
下载: 导出CSV
-
[1] Grund EM, Muise-Helmericks RC. Cost efficient and effective gene transfer into the human natural killer cell line, NK92[J]. J Immunol Methods, 2005, 296(1/2): 31-6. [2] Keller AD, Maniatis T. Identification and characterization of a novel repressor of beta-interferon gene expression[J]. Genes Dev, 1991, 5 (5): 868-79. [3] Mandelbaum J, Bhagat G, Tang HY, et al. Blimp1 is a tumor suppressor gene frequently disrupted in activated B cell-like diffuse large B cell lymphoma[J]. Cancer Cell, 2010, 18(6): 568-79. [4] Grund EM, Muise-Helmericks RC. Cost efficient and effective gene transfer into the human natural killer cell line, NK92[J]. J Immunol Methods, 2005, 296(1/2): 31-6. [5] Turner CJ, Mack DH, Davis MM. Blimp-1, a novel Zinc fingercontaining protein that can drive the maturation of B lymphocytes into immunoglobulin-secreting cells[J]. Cell, 1994, 77(2): 297-306. [6] Huang S. Blimp-1 is the murine homolog of the human transcriptional repressor PRDI-BF1[J]. Cell, 1994, 78(1): 9-13. [7] Mock BA, Liu L, Lepaslier D, et al. The B-lymphocyte maturation promoting transcription factor Blimp1/PRDI-BF1maps to D6S447 on human chromosome 6q21-q22.1 and the syntenic region of mouse chromosome 10[J]. Genomics, 1996, 37(1): 24-8. [8] Gyory I, Wu J, Fejer G, et al. PRDI-BF1 recruits the histone H3 methyltransferase G9a in transcriptional silencing[J]. Nat Immunol, 2004, 5(3): 299-308. [9] Gyory I, Fejer G, Ghosh N, et al. Identification of a functionally impaired positive regulatory domain I binding factor 1 transcription repressor in myeloma cell lines[J]. J Immunol, 2003, 170(6): 3125-33. [10] Lin J, Lwin T, Zhao JJ, et al. Follicular dendritic cellinduced microRNA-mediated upregulation of PRDM1 and downregulation of BCL-6 in non-Hodgkin's B-cell lymphomas[J]. Leukemia, 2011, 25(1): 145-52. [11] Calame K. Activation-dependent induction of Blimp-1[J]. Curr Opin Immunol, 2008, 20(3): 259-64. [12] Tam W, Gomez M, Chadburn A, et al. Mutational analysis of PRDM1 indicates a tumor-suppressor role in diffuse large B-cell lymphomas[J]. Blood, 2006, 107(10): 4090-100. -
点击查看大图
图(2) / 表(1)
计量
- 文章访问数: 664
- HTML全文浏览量: 192
- PDF下载量: 1
- 被引次数: 0
