The correlation between TLR4 expression and apoptosis of hypoxic ischemic brain damage in neonatal rats
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摘要:
目的通过检测TLR4在新生大鼠缺氧缺血性脑损伤（HIBD）中表达变化，并与凋亡情况对比，研究TLR4在新生儿缺氧缺血性脑损伤发病机制中的作用，为临床的进一步研究提供客观的实验依据。 方法选取80只7 d龄新生健康大鼠，随机分为实验组和对照组各40只。TUNEL法检测脑组织细胞凋亡状况，免疫组化SP法检测TLR4表达变化。 结果细胞凋亡缺氧缺血术后6 h阳性率上升，至24 h达到高峰，72 h和7 d表达下降。TlR4实验组于缺氧缺血6 h即出现阳性表达，12 h表达到峰值，24 h无明显变化，72 h和7 d表达下降。实验组与对照组在各时间点比较差异均有统计学意义（P<0.05）。TLR4与细胞凋亡呈正相关（r=0.452）。 结论新生大鼠缺氧缺血性脑损伤组织中TLR4高表达，可能促进细胞凋亡发生，在脑损害的形成过程中起到了重要的作用。 Abstract:Objective To investigate the role of TLR4 in HIBD by observing the change of TLR4 expression and cell apoptosis after hypoxic-ischemic brain damage (HIBD)in a neonatal rat model, and provide an experimental foundation for the futher clinical research. Methods A total of 80 7day postnatal Sprague-Dawley rats were randomly divided into experimental group (n=40) and control group(n=40). The expression of TLR4 was tested by immunohistochemistry, the apoptotic hippocampus cellswere tested by TUNEL. Results The cell apoptosis and the expression of TLR4 increased at HI 6 h, and achieved the hightest increases at HI 12 h, and it continued to maintain the high level in HI 24 h, then decreased at HI 72 h、HI7 d group. There were significant differences of positive rate at different times compared with control group(P<0.05). There was a positive correlation between apoptosis and TLR4(r=0.452) in rats with hypoxic-ischemic brain damage. Conclusion TLR4 over expressed in HIBDcan promote cell apoptosis, and play an important role in the pathogenesis of HIBD. -
Key words:
- neonatal rats /
- hypoxia-ischemia /
- brain damage /
- TLR4 /
- apoptosis
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表 1 实验组与对照组大脑皮质区凋亡细胞阳性率比较(n=40,x±s)
分组 6 h 12 h 24 h 72 h 7 d 对照组 0.2525±0.0423 0.2914±0.0129 0.3132±0.0418 0.2231±0.013 0.1991±0.0594 实验组 0.0518±0.0008* 0.0612±0.0007* 0.0586±0.0005* 0.0561±0.0009* 0.0621±0.0006* t 12.5 8.6 11.42 10.42 9.25 P <0.05 <0.05 <0.05 <0.05 <0.05 
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表 2 实验组与对照组大脑皮质TLR4平均光密度(n=40, x±s)
分组 6 h 12 h 24 h 72 h 7 d 对照组 0.0111±0.0008 0.0105±0.0014 0.0114±0.0007 0.0105±0.0013 0.0112±0.0023 实验组 0.3271±0.0333* 0.4359±0.0428* 0.4342±0.0428* 0.3005±0.0209* 0.2744±0.0288* t 26.86 28.127 27.945 38.974 25.73 P <0.05 <0.05 <0.05 <0.05 <0.05
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