Expression of Fascin in endometrial lesions and its clinicopathological significances
-
摘要:
目的通过检测肌动蛋白聚合蛋白（Fascin）在不同类型子宫内膜病变的表达情况，评估其在子宫内膜增生性病变中的鉴别诊断意义和对子宫内膜样腺癌预后评估的临床价值。 方法应用免疫组化Envision二步法检测50例子宫内膜样腺癌、20例子宫内非典型增生、20例不伴非典型性子宫内膜增生和20例增生期子宫内膜组织中Fascin的表达，并分析Fascin表达程度与子宫内膜样腺癌临床病理参数的相关性。 结果Fascin在增生期子宫内膜组织和不伴非典型性子宫内膜增生组织中均不表达，在子宫内膜非典型增生组织中的少量表达（15%，3/20），但在子宫内膜样腺癌组织中呈弥漫强阳性表达（72%，36/50）。Fascin在子宫内膜样腺癌与其他内膜病变的表达差异有统计学意义（P<0.05）。子宫内膜样腺癌组织中Fascin 的高表达与组织学分级、FIGO分期和肌层浸润深度均密电码切相关，但与淋巴结转移无关（P>0.05）。 结论Fascin在表达有助于子宫内膜样腺癌与其前驱病变的组织学鉴别诊断，并可能通过调控细胞侵袭迁移影响子宫内膜样腺癌的发表和演进。 Abstract:Objective To investigate the expression of Fascin in varied endometrial lesions of uterus and to evaluate itsclinicopathological significances in histoloical diagnosis and prognosis prediction of lesions. Methods Fifty endometrioidadenocarcinomas (EA), 20 atypical hyperplasia of endometrium (AH), 20 endometrial hyperplasia without atypia (HWA) and20 proliferative phase of endometrium (PPE) were collected . Fascin expression was detected in these lesions byimmunohistochemical staining. The relationship between Fascin expression and clinicopathological parameters of lesions wasstatistically analyzed. Results There was no expression of Fascin in PPE and HWA and AH showed Fascin expression (15%, 3/20). Fascin could be detected strongly and diffusely in the most of EA (72%, 36/50). There was a significant difference betweenEA and other lesions in Fascin expression (P<0.05). In EAs, Fascin expression was closely correlated with tumor histologicalgrading, FIGO staging, and depth of myometrial invasion, respectively. There was no correlation found between Fascinexpression and lymph node metastasis (P>0.05). Conclusion Fascin might be an important molecular marker for differentialdiagnosis between EA and its precursor lesions. Furthermore, Fascin might play a critical role in development and progressionof ES by modulating tumor cell invasion and migration. -
Key words:
- Endometrioid adenocarcinoma /
- Fascin /
- differential diagnosis /
- prognosis
-
图 1 Fascin在子宫内膜组织中的免疫组织化学染色表达（×400）
A: 示正常增生期子宫内膜腺上皮阴性表达, 但在微血管内皮细胞及间质细胞中表达; B: 示子宫内膜增生，增生上皮呈阴性表达，但在微血管内皮细胞及间质细胞中表达; C:示子宫内膜非典型增生, 增生的异型上皮细胞胞质及胞膜呈局灶阳性表达而且染色较弱; D:示子宫内膜样腺癌, 癌细胞胞质及胞膜染色深而且呈弥漫阳性表达.
表 1 Fascin表达与子宫内膜病变特征的关系（n）
项目 n 阴性 阳性 阳性率(%) P 正常增生期子宫内膜 20 20 0 0 <0.0001 子宫内膜增生 20 20 0 0 <0.0001 子宫内膜非典型增生 20 17 3 15 0.0013 子宫内膜样腺癌 50 21 29 58 FIGO分期 0.0255 Ⅰ 18 12 6 33.3 Ⅱ 15 5 10 66.7 Ⅲ-Ⅳ 17 4 13 76.5 组织分级 0.0155 G1 24 15 9 37.5 G2 14 4 10 71.4 G3 12 2 10 83.3 肌层浸润深度 0.0223 <1/2 34 18 16 47 ≥1/2 16 3 13 81.2 有无淋巴结转移 0.2878 无 2 19 23 54.8 有 8 2 6 75 
下载: 导出CSV
-
[1] Siegel R, Naishadham D, Jemal A. Cancer statistics, 2013[J]. CA Cancer J Clin, 2013, 63(1): 11-30. doi: 10.3322/caac.v63.1 [2] Jeong NH, Lee JM, Lee SK. Current status in the management of uterine corpus cancer in Korea[J]. J Gynecol Oncol, 2010, 21(3): 151-62. doi: 10.3802/jgo.2010.21.3.151 [3] Stewart CJ, Crook ML, Leung YC, et al. Expression of cell cycle regulatory proteins in endometrial adenocarcinoma: variations in conventional tumor areas and in microcystic, elongated and fragmented glands[J]. Mod Pathol, 2009, 22(5): 725-33. doi: 10.1038/modpathol.2009.33 [4] Köbel M, Langhammer T, Hűttelmaier S, et al. Ezrin expression is related to poor prognosis in FIGO stage I endometrioid carcinomas [J]. Mod Pathol, 2006, 19(4): 581-7. doi: 10.1038/modpathol.3800567 [5] Salvesen HB, Akslen LA. Molecular pathogenesis and prognostic factors in endometrial carcinoma[J]. APMIS, 2002, 110(10): 673-89. doi: 10.1034/j.1600-0463.2002.1101001.x [6] Jayo A, Parsons M. Fascin: a key regulator of cytoskeletal dynamics [J]. Int J Biochem Cell Biol, 2010, 42(10): 1614-7. doi: 10.1016/j.biocel.2010.06.019 [7] Yamashiro S, Yamakita Y, Ono S, et al. Fascin, an actin-bundling protein, induces membrane protrusions and increases cell motility of epithelial cells[J]. Mol Biol Cell, 1998, 9(5): 993-1006. doi: 10.1091/mbc.9.5.993 [8] Schäfer C, Faust U, Kirchgessner N, et al. The filopodium: a stable structure with highly regulated repetitive cycles of elongation and persistence depending on the actin cross-linker fascin[J]. Cell Adh Migr, 2011, 5(5): 431-8. doi: 10.4161/cam.5.5.17400 [9] Zhang FR, Tao LH, Shen ZY, et al. Fascin expression in human embryonic, fetal, and normal adult tissue [J]. J Histochem Cytochem, 2008, 56(2): 193-9. doi: 10.1369/jhc.7A7353.2007 [10] Swierczynski SL, Maitra A, Abraham SC, et al. Analysis of novel tumor markers in pancreatic and biliary carcinomas using tissue microarrays[J]. Hum Pathol, 2004, 35(3): 357-66. doi: 10.1016/j.humpath.2003.10.012 [11] Crothey A, Hashizume R, Sahin AA, et al. Fascin,an actinbundling protein associated with cell motility,is upregulated in hormone receptor negative breast Cancer[J]. Br J Cancer, 2000, 83(5): 870-3. http://cn.bing.com/academic/profile?id=1493334187&encoded=0&v=paper_preview&mkt=zh-cn [12] Hu W, Mecrea PD, Deavers M, et al. Increased expression of fascin [J]. motility associated protein,in cell cultures derived from ovariancancer and in borderline and carcinomatous ovarian tumors. ClinExp Metastasis, 2000, 18(7): 83-8. http://cn.bing.com/academic/profile?id=217939331&encoded=0&v=paper_preview&mkt=zh-cn [13] Pelosi G, Pastorino U, Pasini F, et al. Independent prognostic value of fascin immunoreactivity in stage I nonsmall cell lung cancer[J]. Br J Cancer, 2003, 88(4): 537-47. doi: 10.1038/sj.bjc.6600731 [14] Hashimoto Y, Ito T, Inoue H, et al. Prognostic significance of fascin overexpression in human esophageal squamous cell carcinoma[J]. Clin Cancer Res, 2005, 11(7): 2597-605. doi: 10.1158/1078-0432.CCR-04-1378 [15] Hashimoto Y, Kim DJ, Adams JC. The roles of fascins in health and disease[J]. J Pathol, 2011, 224(3): 289-300. doi: 10.1002/path.v224.3 [16] 黄岭, 马晓欣, 舒红. fascin在子宫内膜病变中的表达及意义[J]. 中国现代医学杂志, 2008, 18(2): 198-201. http://www.cnki.com.cn/Article/CJFDTOTAL-ZXDY200802020.htm [17] Kabukcuoglu S, Oner U, Ozalp SS, et al. Prognostic significance of fascin expression in endometrioid carcinoma[J]. Eur J Gynaecol Oncol, 2006, 27(5): 481-6. http://cn.bing.com/academic/profile?id=32572885&encoded=0&v=paper_preview&mkt=zh-cn -
点击查看大图
图(1) / 表(1)
计量
- 文章访问数: 646
- HTML全文浏览量: 175
- PDF下载量: 1
- 被引次数: 0
