Effect of metacortandracin administration method and time on bone mineral density in rats
-
摘要:
目的 观察肌肉注射、口服给予强的松的方法对大鼠骨密度、血清指标的影响。 方 法将45只雄性SD大鼠随机分为3组 （正常组15只、口服组15只、肌肉注射组15只），其中正常组大鼠作为阴性对照，不给予任何药物干预；口服组大鼠给予强的松 10 mg/（kg· d）灌胃；肌肉注射组大鼠给予强的松10 mg/（kg· 2 d）。观察给药12周后大鼠椎骨骨密度水平、血清钙、磷、碱性磷酸 酶的变化。 结果12周后，与正常组相比，口服组、肌肉注射组大鼠椎骨骨密度、血钙水平均显著下降（P＜0.05），碱性磷酸酶、血 磷水平均显著升高。此外，肌肉注射组与口服组大鼠椎骨骨密度水平相比下降（P＜0.05），血清钙、磷、碱性磷酸酶水平相比则无 明显差异（P＞0.05）。 结论与正常组相比较，口服和肌肉注射强的松都能降低大鼠椎骨的骨密度值，同时对大鼠血清钙、磷、碱性 磷酸酶有一定的影响，该结论可为需长期使用激素的骨质疏松症患者临床安全合理用药提供相关实验依据。 Abstract:ObjectiveTo observe the effect of metacortandracin administration method and time on bone metabolism in rats. Methods A total of 45 SPF rats were randomly divided into three groups (Normal group Intragastric administration group Injection group). Normal group, as a control group, administrated with nothing, Intragastric administration group with prednisone 10 mg/(kg · d). Injection group with prednisone 10 mg/(kg · 2d) , which were observed 12 weeks. All rat s were examined for bone mineral density (BMD), serum calcium，phosphorus, alkaline phosphatase. Resultsafter 12th weeks, compared with Normal group, BMD and serum calcium decreased significantly while the phosphorus, alkaline phosphatase increased obviously in Intragastric administration group and Injection group (P＜0.05). Compared with Intragastric administration group, BMD decreased significantly in Injection group (P＜0.05) while the level of serum calcium, phosphorus, alkaline phosphatase were not changed significantly (P＞0.05). ConclusionCompared with Normal group, both Intragastric administration and injection with prednisone could influence the level of BMD, serum calcium, phosphorus, alkaline phosphatase, while injection with prednisone could decrease the BMD more significantly, the research result shows some meaningful theroptic methods for the op patients. -
Key words:
- administration method /
- metacortandracin /
- rats /
- bone mineral density /
- calcium /
- phosphorus /
- alkaline phosphatase
-
表 1 12周后血清钙(Ca)、磷(P)、碱性磷酸酶(ALP)测定结果（n=15）
分组 Ca P ALP 正常组 2.91±0.14 1.69±0.06 92.56±6.4 肌肉注射组 2.35±0.11ac 2.09±0.1ac 181.65±8.5ac 口服组 2.37±0.09a 2.11±0.09a 182.27±0.09a 与正常组相比，aP＜0.05；与口服组相比，cP＞0.05. 
下载: 导出CSV
-
[1] Miron RJ, Saulacic N, Buser D, et al. Osteoblast proliferation and differentiation on a barrier membrane in combination with BMP2 and TGFβ1[J]. Clin Oral Investig, 2013, 17(3): 981-8. doi: 10.1007/s00784-012-0764-7 [2] Bar- Shavit Z. The osteoclast: a multinucleated, hematopoietic origin, bone-resorbing osteoimmune cell [J]. J Cell Biochem, 2007, 102 (5): 1130-9. doi: 10.1002/(ISSN)1097-4644 [3] Thawani JP, Wang AC, Than KD, et al. Bone morphogenetic proteins and cancer: review of the literature[J]. Neurosurgery, 2010, 66 (2): 233-46; discussion 246. doi: 10.1227/01.NEU.0000363722.42097.C2 [4] 张凤丽, 邢小平. 糖皮质激素所致骨质疏松症的临床研究进展[J]. 国际内分泌代谢杂志, 2007, 27(1): 62-4. [5] Vestergaard P, Rejnmark L, Mosekilde L. Fracture risk associated with systemic and topical corticosteroids[J]. Intern Med, 2005, 257 (4): 374-84. doi: 10.1111/jim.2005.257.issue-4 [6] 廖二元. 糖皮质激素所致骨质疏松症的预防和治疗[J]. 药品评价, 2012, 9(7): 28-35. http://www.cnki.com.cn/Article/CJFDTOTAL-YPPJ201207007.htm [7] van Everdingen AA, Siewertsz van Reesema DR, Jacobs JW, et al. Low- dose glucocorticoids in early rheumatoid arthritis: discordant effects on bone mineral density and fractures[J].. Clin Exp Rheumatol, 2003, 21(2): 155-60. [8] Kelly A, Bartholom EP, Lapwort HA, et al. Peripher lbone density in patients with rheumatoid arthritis and factors which influence it [J]. Eur J Int Med, 2002, 13: 423. doi: 10.1016/S0953-6205(02)00129-2 [9] Kirwan JR. The effect of glucocorticoids on joint destruction in rheumatoid arthritis. The arthritis and rheumatism council low-dose glucocorticoid study group[J]. N Engl J Med, 1995, 333(3): 142. doi: 10.1056/NEJM199507203330302 [10] Hall GM, Spector TD, Griffin AJ, et al. The effect of rheumatoid arthritis and steroid therapy on bone density in post menopausal women [J]. Arthritis Rheum, 1993, 36(11): 1510. doi: 10.1002/(ISSN)1529-0131 [11] Lane NE, Pressman AR, Star VL, et al. Rheumatoid arthritis and bone mineral density in elderly women. The Study of Osteoporotic Fractures Research Group[J]. J Bone Miner Res, 1995, 10(2): 257- 63. [12] 高泉, 周磊, 高波. 糖皮质激素的不同给药方法对大鼠骨密度的影响[J]. 实用临床医药杂志, 2012, 16(5): 4-6. http://www.cnki.com.cn/Article/CJFDTOTAL-XYZL201205001.htm [13] 吴天秀, 王宏芬, 谢华, 等. 骨形态计量学观察糖皮质激素的不同剂量和不同的给药方法对大鼠松质骨的影响[J]. 国际医药卫生导报, 2006, 12(4): 7-9. http://www.cnki.com.cn/Article/CJFDTOTAL-GJYW200604001.htm [14] 陈新军, 霍维玲, 李香业, 等. 联合检测尿脱氧吡啶啉与骨源性碱性磷酸酶诊断骨质疏松症[J]. 临床检验杂志, 2006, 24(4): 253-4. http://www.cnki.com.cn/Article/CJFDTOTAL-LCJY200604009.htm [15] Pederson L, Kremer M, Foged NT, et al. Evidence of a correlation of estrogen receptor level and avian osteoclast estrogen responsiveness [J]. J Bone Miner Res, 1997, 12(5): 742-52. doi: 10.1359/jbmr.1997.12.5.742 [16] 胡稷杰, 刘亚伟, 何敏毅, 等. LASP1 下调和ferritin 上调在rhBMP-2 诱导比格犬BMSCs成骨分化中起重要作用[J]. 南方医科大学学报, 2013, 33(8): 1207-9. http://www.cnki.com.cn/Article/CJFDTOTAL-DYJD201308025.htm [17] 王义生, 张振, 张鑫, 等. 乙醇对兔骨髓间充质干细胞6种神经递质 mRNA表达的影响[J]. 郑州大学学报:医学版, 2011, 46(3): 357-8. http://www.cnki.com.cn/Article/CJFDTOTAL-HNYK201103011.htm [18] 迭小红, 罗庆, 陈聪, 等. 骨形态发生蛋白9、6 双表达腺病毒载体的构建及其成骨诱导作用[J]. 南方医科大学学报, 2013, 33(9): 1273-5. http://www.cnki.com.cn/Article/CJFDTOTAL-DYJD201309005.htm [19] 王义生, 王小刚, 李月白, 等. 靶向PPARC基因RNA干扰对激素抑制家兔骨髓基质细胞成骨分化的影响[J]. 郑州大学学报: 医学版, 2009, 44(2): 275-6. http://cdmd.cnki.com.cn/Article/CDMD-10459-2010052086.htm [20] Ledger GA, Burritt MF, Kao PC, et al. Abnormalities of parathyroid hormone secretion in elderly women that are reversible by short term therapy with 1,25-dihydroxyvitamin D3[J]. J Clin Endocrinol Metab, 1994, 79(1): 211-6. http://cn.bing.com/academic/profile?id=49ebfecf98907a80ebc211c9db7539e3&encoded=0&v=paper_preview&mkt=zh-cn [21] Hyung-Young H. Yoon Ye-Yeon won,Yoon-Sok chung[J]. Poncirin prevents bone loss in glucocorticoid- induced osteoporosis in vivo and in vitro[J]. J Bone Miner Metab, 2012, 30: 509-16. http://cn.bing.com/academic/profile?id=19b14b3010dc09407745501f0a981935&encoded=0&v=paper_preview&mkt=zh-cn -
点击查看大图
表(1)
计量
- 文章访问数: 593
- HTML全文浏览量: 286
- PDF下载量: 3
- 被引次数: 0
