Abstract: Prostate cancer is a serious threat to men's health. In recent years, the incidence of prostate cancer in my country has also increased rapidly. Early detection of prostate cancer has a very positive significance for improving the survival rate. At present, the prostate cancer guidelines still need to be diagnosed by ultrasound-guided prostate biopsy, and then treated by means of active monitoring, radical resection, radiotherapy, and local radiotherapy. However, prostate biopsy increases unnecessary risks such as urinary retention, hematuria, and misses up to one-third of cancer tissues. The current treatment methods lack specificity and over-or under-treatment of patients. In recent years, the diagnosis and treatment of prostate cancer have entered the molecular level, and the non-invasive and accurate diagnosis and treatment of prostate cancer through molecular imaging has shown great development prospects. This article reviews and summarizes the application progress in prostate cancer of four common molecular imaging technologies, nuclear medicine molecular imaging, MR molecular imaging, optical molecular imaging, and ultrasound molecular imaging.