An animal imaging study of a novel magnetic resonance contrast agent
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摘要:
目的 研究一种新型含钆磁共振对比剂（Gd-PPF-S-CAs）在小鼠体内正常器官的成像研究。 方法 首先将Gd-PPF-S-CAs与小分子磁共振对比剂（DTPA-Gd）在体外弛豫效能进行对比；其次选取正常Balb/C小鼠10只，随机分为两组，一组注射Gd-PPF-S-CAs，一组注射临床用DTPA-Gd，分别进行小鼠的肝、肾、膀胱扫描，将Gd-PPF-S-CAs组在体内增强效果、增强幅度以及强化持续时间3个方面与临床DTPA-Gd进行对比。 结果 体外弛豫效能结果显示，实验组Gd-PPF-S-CAs弛豫率为15.43 mmol-1s-1，是临床DTPA-Gd弛豫率（3.53 mmol-1s-1）的5倍；通过对小鼠肝、肾、膀胱的增强效果、增强幅度以及强化持续时间进行分析，Gd-PPF-S-CAs较临床用DTPA-Gd在小鼠肝、肾、膀胱有着更为明显的增强效果、较高的增强幅度以及长时间的强化持续窗口。 结论 Gd-PPF-S-CAs在体外有着较高的弛豫效能；在小鼠体内正常器官有着明显的增强效果和长效的强化持续时间，能够有效的解决小分子临床DTPA-Gd的增强幅度较低、组织对比度不高和成像窗口时间较短的不足；同时，Gd-PPF-S-CAs具有酶降解特性，能够在体内快速代谢，有效地解决了Gd3+对比剂的潜在毒性问题，具有良好的临床应用前景。 -
关键词:
- Gd-PPF-S-CAs /
- 磁共振对比剂 /
- 小分子磁共振对比剂
Abstract:Objective To explore the imaging of a novel gadolinium-containing magnetic resonance contrast agent (Gd-PPF-S-CAS) in normal organs in mice. Methods Firstly, the relaxation performance of GD-PPF-S-CAS and DTPA-GD in vitro was compared. Secondly, 10 normal Balb/C mice were randomly divided into two groups. One group was injected with Gd-PPF-S-CAs, and the other was injected with clinical DTPA-Gd. The liver, kidney and bladder of the mice were scanned respectively. The Gd-PPF-S-CAs group was compared with clinical DTPA-Gd in terms of in vivo enhancement effect, enhancement amplitude and duration of enhancement. Results The relaxation rate of Gd-PPF-S-CAs in the experimental group was 15.43 mmol-1s-1, which was five times that of the clinical DTPA-Gd relaxation rate of 3.53 mmol-1s-1. By analyzing the enhancement effect, amplitude and duration of liver, kidney and bladder in mice, GD-PPF-S-CAS had more obvious enhancement effect, higher enhancement amplitude and longer duration of enhancement than DTPA-Gd in mice liver, kidney and bladder. Conclusions Gd-PPF-S-CAs have a high relaxation effect in vitro. Normal organs in mice have obvious enhancement effects and long-lasting enhancement duration, which can effectively solve the shortcomings of small molecular DTPA-Gd enhancement, low tissue contrast and short imaging window time. At the same time, Gd-PPF-S-CAs have the characteristics of enzymatic degradation and can be metabolized in the body quickly, which effectively solves the potential toxicity of Gd3++ contrast agent and has good clinical application prospects. -
Key words:
- Gd-PPF-S-CAs /
- magnetic resonance imaging contrast agents /
- DTPA-Gd
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图 1 3.0 T磁共振测定纵向弛豫率
A: T1加权图像; B: r1值.
Figure 1. Measurement of longitudinal relaxivity by 3.0 T MR.
图 2 注射Gd-PPF-S-CAs后主要器官（肝、肾和膀胱）的MRI
A: T1加权图像; B:相对信号增强比值.
Figure 2. MRI of major organs (liver, kidney and bladder) after Gd-PPF-S-CAs injection.
图 3 MRI of major organs (liver, kidney and bladder) after DTPA-Gd injection.
A: T1加权图像; B:相对信号增强比值.
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