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Volume 40 Issue 2
May  2018
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Article Navigation >  Journal of Molecular Imaging  > 2017  >  40(2): 202-206
Jizhou WANG, Yu ZENG, Ye SONG, Songtao QI. Effect of different passage number on the biological characteristics of U87 glioblastoma cell line[J]. Journal of Molecular Imaging, 2017, 40(2): 202-206. doi: 10.3969/j.issn.1674-4500.2017.02.22
Citation: Jizhou WANG, Yu ZENG, Ye SONG, Songtao QI. Effect of different passage number on the biological characteristics of U87 glioblastoma cell line[J]. Journal of Molecular Imaging, 2017, 40(2): 202-206. doi: 10.3969/j.issn.1674-4500.2017.02.22
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Effect of different passage number on the biological characteristics of U87 glioblastoma cell line

doi: 10.3969/j.issn.1674-4500.2017.02.22

  • Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China

  • Received Date: 2017-02-06
  • Publish Date: 2017-04-01
    Abstract
  • Objective To unravel the differences of biological characteristics and the uderlying molecular mechanisms between different passages of U87 glioblastoma cell line. Methods U87 (I) and U87 (II), with less or more passage number respectively, were established examined separately by MTT assay, transwell chamber assay, boyden chamber assay. Western blot was used to analyze the expression of CTHRC1, FOXM1, PLOD2, MMP9, TGF-β, E-cadherin, Slug, Snail, Vimentin, PI3K, p-PI3K, Akt and p-Akt separately in these two types of cells. Results Compared to U87 (II), U87 (I) was more prone to form tubules and showed more invasiveness, while there were no differences between U87 (I) and U87 (II) in proliferation and migration. The expression of markers of EMT varies in these two types of cells, with more expression of E-cadherin and Slug and less expression of Vimentin and Snail in U87 (II). Moreover, U87 (II) was found to have greater amount of Akt and p-Akt, but have smaller amount of p-PI3k. No significant difference was found on the expression of PI3K between these cells. Additionally, the expression of PLO2, CTHRC1 and MMP9 were higher in U87 (I) than that in U87 (II), while the expression of TGF-β in U87 (I) was lower than that in the latter one. Conclusion As the passage number increased, U87 cell lines exhibited changes in invasiveness as well as some oncogenic genes’ expressions. It may lead to the different results during different periods, making the results inconvincible. Together, our results showed that, to lessen the influence of passage numbers on cells’ biological characteristics, scientists should use cells with same passage numbers and finish the experiments as quickly as they can.

     

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