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Volume 39 Issue 3
Feb.  2017
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Article Navigation >  Journal of Molecular Imaging  > 2016  >  39(3): 292-296
Xiaoming OUYANG, Cheng ZHI, Yun ZHOU, Degui LIAO, Miaoling LAI. Effects of astragalus injection on the expression of β-Catenin and E-cadherin in human endometrial carcinoma cells[J]. Journal of Molecular Imaging, 2016, 39(3): 292-296. doi: 10.3969/j.issn.1674-4500.2016.03.28
Citation: Xiaoming OUYANG, Cheng ZHI, Yun ZHOU, Degui LIAO, Miaoling LAI. Effects of astragalus injection on the expression of β-Catenin and E-cadherin in human endometrial carcinoma cells[J]. Journal of Molecular Imaging, 2016, 39(3): 292-296. doi: 10.3969/j.issn.1674-4500.2016.03.28
shu

Effects of astragalus injection on the expression of β-Catenin and E-cadherin in human endometrial carcinoma cells

doi: 10.3969/j.issn.1674-4500.2016.03.28
  • 1.

    Department of Pathology, Guangzhou Medical University, Guangzhou 510260, China

  • 2.

    Clinical Laboratory Second Affiliated Hospital, Guangzhou Medical University, Guangzhou 510260, China

  • Received Date: 2016-04-15
  • Publish Date: 2016-07-20
    Abstract
  • Objective To investigate the effects of astragalus injection on the mRNA and protein expression of β-catenin and E-cadherin in HEC-1-Bendometrial carcinoma cells. Methods Cell climbing slices of HEC-1-B cells were prepared for the intervening experiment of astragalus injection on endometrial carcinoma cells, then their mRNA and protein expression of β-catenin and E-cadherin detected by RT-PCR and immunohistochemistry. Results RT-PCR results showed that with the incubation time of endometrial cancer cells in conditioned medium containing astragalus injection increased, E-cadherin gene expression of the cells gradually increased (P＜0.05), while the β-catenin gene expression gradually decreased (P＜0.05). The immunocytochemistry result indicated thatastragalus could significantly decrease β-catenin expression and increase E-cadherin expression in HEC-1-B cells（P＜0.05）. Moreover, accompanied by elevated concentration and treatment time of astragalus injection, β-catenin expression dropped progressively (P＜0.05), meanwhile with the concentration of astragalus injection raised, E-cadherin expression gradually increased (P＜0.05). Conclusion Astragalus injection could significantly inhibit WNT signaling pathway by promoting mRNA andprotein expression of E-cadherin,decreasing mRNA and protein expression ofβ-cateninin a dose- and time-dependent manner, which might be one of the mechanisms for its anti-tumor effect.

     

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