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Volume 37 Issue 3
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Article Navigation >  Journal of Molecular Imaging  > 2014  >  37(3): 135-139
Shuhua LI, Tingting CHEN, Yukun LIU, Hao LI, Yiying CHENG, Yajie ZHANG. Construct ion of recombinant adenovirus vector pDC316-hIL-24 and the detection of viral titer[J]. Journal of Molecular Imaging, 2014, 37(3): 135-139. doi: 10.3969/j.issn.1674-4500.2014.03.01
Citation: Shuhua LI, Tingting CHEN, Yukun LIU, Hao LI, Yiying CHENG, Yajie ZHANG. Construct ion of recombinant adenovirus vector pDC316-hIL-24 and the detection of viral titer[J]. Journal of Molecular Imaging, 2014, 37(3): 135-139. doi: 10.3969/j.issn.1674-4500.2014.03.01
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Construct ion of recombinant adenovirus vector pDC316-hIL-24 and the detection of viral titer

doi: 10.3969/j.issn.1674-4500.2014.03.01

  • Guangzhou Medical University, Guangzhou 510182, China

  • Received Date: 2014-04-19
  • Publish Date: 2014-07-01
    Abstract
  • ObjectiveTo onstruct recombinant adenovirus vector pDC316-hIL-24 and detect the viral titer.MethodsTotal mRNA was ex tracted from HEK293 cells, and specific primer pairs were designed in order to clone human interleukin-24 (hIL-24) gene cDNA by RT-PCR method. hIL-24 gene was directional constructed into shuttle plasmidr (pDC316-EGFP) after digestion by restrictive endoenzyme BglⅡ, and HindⅢ. HEK293 cells were co-transfected with the constructed recombinant shuttle plasmid pDC316-EGFP and large adenovirus helper plasmid pBHGlox (delta) E1, 3Cre in mediation of liposome. The constructed recombinant adenovirus vector was named pDC316-hIL-24. Recombinant adenovirus was amplificated and purificated in HEK293 ce lls.ResultsRecombinant adenovirus vector pDC316-hIL-24 was constructed successfully and high titer recombinant adenovirus was obtained.ConclusionThe construction of recombinant adenovirus vector pDC316-hIL-24 and the obtain of recombinant adenovirus will lay down the basis for developing gene therapy of cancer.

     

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