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Volume 42 Issue 1
Jan.  2019
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Article Navigation >  Journal of Molecular Imaging  > 2019  >  42(1): 67-69
Le HU, Yuyang LI, Zhiyong KE, Yue ZHANG, Wuju ZHANG. A preliminary study of conditional limb bud mesenchymal stem cell FKBP38 knockout mice[J]. Journal of Molecular Imaging, 2019, 42(1): 67-69. doi: 10.12122/j.issn.1674-4500.2019.01.16
Citation: Le HU, Yuyang LI, Zhiyong KE, Yue ZHANG, Wuju ZHANG. A preliminary study of conditional limb bud mesenchymal stem cell FKBP38 knockout mice[J]. Journal of Molecular Imaging, 2019, 42(1): 67-69. doi: 10.12122/j.issn.1674-4500.2019.01.16
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A preliminary study of conditional limb bud mesenchymal stem cell FKBP38 knockout mice

doi: 10.12122/j.issn.1674-4500.2019.01.16
  • 1.

    School of Basic Medical Science, Southern Medical University, Guangzhou 510515, China

  • 2.

    Guangdong Zhongyuan High School, Guangzhou 511400, China

  • Received Date: 2018-11-04
  • Publish Date: 2019-01-01
    Abstract
  • Objective To construct and identify conditional limb bud mesenchymal stem cell FKBP38 knockout mice, and provide an animal model for the mechanism of FKBP38 gene in bone development and osteoarthritis. Methods Prrx1-Cre mice were mated with FKBP38flox/flox to produce F1 mice. The genotype was Prrx1-Cre(Fkbp38flox/+) mice. The mice with genotype Prrx1-Cre(Fkbp38flox/+) were selected. FKBP38flox/flox mice were crossed to obtain F2 mice. Mice with genotype Prrx1-Cre(Fkbp38flox/flox) were used to construct model mice for the experiment. The mouse genotype was identified at 3 weeks of age. The knockdown effect of FKBP38 gene was verified by Western blot at 4 weeks of age. Results F2 mice contained knockout mice. The PCR results showed that the genotype was Cre/FKBP38flox/flox. The results of Western blot showed that the FKBP38 gene knockout effect was obvious. Conclusion Based on the Cre/loxp system, the limb bud mesenchymal stem cell FKBP38 knockout mouse is propagated to provide a model basis for exploring the mechanism of FKBP38 in bone development and osteoarthritis at the animal level.

     

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