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摘要:
目的 研究TPX2基因对乳腺癌干细胞活性及放疗敏感性的影响。 方法 使用蛋白质印迹法检测TPX2基因在乳腺癌细胞及组织中的表达情况。使用RT-PCR检测TPX2基因在乳腺癌组织及癌旁组织中的表达差异。使用慢病毒干扰载体建立稳定低表达TPX2蛋白的乳腺癌细胞。平板克隆、MTT试验验证TPX2对乳腺癌细胞生长能力的影响。流式细胞仪检测敲除TPX2之后对乳腺癌细胞凋亡比率的影响。检测CD44阳性细胞比例、肿瘤球形成能力以及侧群细胞比例，验证敲除TPX2之后对乳腺癌干细胞活性影响。 结果 TPX2基因在乳腺癌组织及细胞中表达水平明显升高（P<0.05）。敲除TPX2之后，可以使乳腺癌细胞生长能力减弱并促进细胞凋亡。TPX2敲除后，可以降低乳腺癌干细胞活性及提高放疗敏感性。 结论 TPX2为促进乳腺癌细胞生长的一个癌基因，其可通过提高乳腺癌干细胞活性来影响乳腺癌细胞的放疗敏感性。 -
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