Expression and significance of CD8 + CD28 - T cells for patients with interstitial lung disease secondary to rheumatoid arthritis who underwent methylprednisolone impact therapy
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摘要:
目的探讨类风湿关节炎（RA）继发肺间质病变（ILD）患者激素冲击治疗前后CD8+CD28-T细胞的变化与意义 方法共入选23例RA-ILD患者（A组），选取同期就诊的RA患者25例（B组）、非RA致ILD患者25例（C组）、25例健康人（D组）作为对照。对以上具有甲泼尼龙冲击适应症的A组患者进行治疗，治疗前后采用流式细胞术检测上述4组外周血CD8+CD28-T细胞及其细胞因子白介素10（IL-10）和转化生长因子β1（TGF-β1）的百分含量变化。 结果与D组比较，A、B及C三组的CD8+CD28-T细胞、IL-10及TGF-β1均明显升高，含量由高至低均表现为A>C>B>D，且四组间任意两组上述三项指标均有统计学差异（P<0.05）。治疗后A组患者CD8+CD28-T细胞、IL-10及TGF-β1均较治疗前明显降（P<0.05）。 结论CD8+CD28-T细胞在类风湿关节炎继发肺间质病变发病过程可能起到重要作用，而甲泼尼龙可显著抑制该细胞活性，有望作为结缔组织病致肺间质病变的病情观察及疗效评价指标。 -
关键词:
- 类风湿关节炎 /
- 继发肺间质病变 /
- 激素冲击治疗 /
- CD8+CD28-T细胞 /
- 细胞因子
Abstract:Objective To investigate the rheumatoid arthritis (RA) patients with secondary pulmonary interstitial disease (ILD) hormone impact CD8 + CD28 -T cells before and after the treatment with the change of meaning. Methods Included in the total of 23 patients with RA-ILD (group A), selection of RA patients during the same period of 25 cases (group B), the RA patients with ILD 25 cases (group C), 25 cases of healthy people (group D) as A control. For more than A group of patients with A prednisolone impact indications for treatment, using flow cytometry to detect the above four groups before and after the treatment of peripheral blood CD8 + CD28 - T cells and cytokines interleukin 10 (IL-10), and transforming growth factor beta 1 (TGF - beta 1) the percentage of change. Results Compared with group D, A, B and C three groups of CD8 + CD28 - T cells, IL-10 and TGF - beta 1 were significantly increased, content from high to low were characterized by A>C>B>D, and four groups between any two sets of the above three indicators are statistically significant (P<0.05). A group of patients after treatment of CD8 +CD28 - T cells, IL-10 and TGF-beta 1 than before treatment significantly reduced (P<0.05). Conclusion CD8 + CD28 - T cells on the pathogenesis of rheumatoid arthritis secondary pulmonary interstitial lesions process may play an important role, and methyl prednisolone can significantly inhibit the cell activity, is expected to be as a connective tissue disease caused by pulmonary interstitial lesions was observed and curative effect evaluation index. -
表 1 四组观察对象CD8+CD28-T细胞及其细胞因子百分含量比较(x±s，%)
分组 n CD8+CD28-T细胞 IL-10 TGF-β1 A 23 8.96±3.82*# 6.53±2.45*# 4.27±1.16# B 25 5.38±2.97# 4.09±1.87*# 3.12±0.95*# C 25 6.49±2.72*# 5.84±2.19*# 2.37±1.39*# D 25 3.17±0.96* 2.53±0.72* 1.54±0.49* F 4.821 3.724 2.537 P 0.019 0.023 0.036 与B组比较,*P<0.05; 与D组比较,#P<0.05. 
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表 2 治疗前后RA-ILD患者CD8+CD28-T细胞及其细胞因子百分含量比较(n=23，x±s，%)
治疗前后 CD8+CD28-T细胞 IL-10 TGF-β1 前 8.47±3.69 6.33±2.29 4.38±1.36 后 5.26±1.17 3.87±1.04 2.04±0.76 t 3.182 2.761 2.516 P 0.026 0.031 0.039
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