-
摘要:
目的探讨脓毒症大鼠心肌损伤病理学及肌钙蛋白的改变及其意义。 方法将20只SD大鼠随机分为内毒素组与对照组各10只，前者经尾静脉注射内毒素7 mg/kg，对照组注射等量生理盐水；药物注射后24、48 h分别处死动物，心肌经过处理后，进行病理学镜下形态观察。并通过PCR方法检测心肌细胞cTnI表达量。 结果内毒素组大鼠24 h后心肌细胞缩小，心肌纤维转疏松，注射内毒素48 h后心肌细胞周围可见淋巴细胞增多，心肌细胞间隙可见血管扩张，内有红细胞。内毒素注射后24、48 h心肌细胞cTnI表达量明显降低。 结论内毒素血症可引起大鼠心肌细胞病理学改变及cTnI表达降低，是脓毒症大鼠的重要病理生理学改变。 -
图 1 两组大鼠心肌细胞HE染色比较图
1A: 注射NS大鼠×100/HP；1B：注射NS大鼠×400/HP; 2A ×100/HP,2B: ×400/HP: 注射内毒素24 h后,大鼠心肌细胞疏松,心肌纤维变细,血管扩张,内见红细胞; 3A ×100/HP,3B ×400/HP: 注射内毒素48 h后,大鼠心肌细胞疏松,心肌纤维变细,血管扩张,细胞间隙可见较多炎症细胞侵润.
-
[1] Hotchkiss RS, Irene EK. The pathophysiology and treatment of sepsis[J]. N Engl J Med, 2003, 348(2): 138-50. doi: 10.1056/NEJMra021333 [2] Artigas A, Niederman MS, Torres A, et al. What is next in sepsis: current trials in sepsis[J]. Expert Rev Anti Infect Ther, 2012, 10(8): 859-62. doi: 10.1586/eri.12.82 [3] Vieillard-Baron A, Caille V, Charron CA, et al. Actual incidence of global left ventricular hypokinesia in adult septic shock[J]. Crit Care Med, 2008, 36(6): 1701-6. doi: 10.1097/CCM.0b013e318174db05 [4] Celes MR, Prado CM, Rossi MA. Sepsis: going to the heart of the matter[J]. Pathobiology, 2013, 80(2): 70-86. doi: 10.1159/000341640 [5] Livak KJ, Schmittgen TK, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2[Z], 2001: 402-8. [6] Avlas O, Fallach R, Shainberg A, et al. Toll-like receptor 4 stimulation initiates an inflammatory response that decreases cardiomyocyte contractility[J]. Antioxid Redox Signal, 2011, 15(7): 1895-909. doi: 10.1089/ars.2010.3728 [7] Fallach R, Shainberg A, Avlas O, et al. Cardiomyocyte toll-like receptor 4 is involved in heart dysfunction following septic shock or myocardial ischemia[J]. J Mol Cell Cardiol, 2010, 48(6): 1236-44. doi: 10.1016/j.yjmcc.2010.02.020 [8] Duncan DJ, Hopkins PM, Harrison SM. Negative inotropic effects of tumour necrosis factor-alpha and interleukin-1beta are ameliorated by alfentanil in rat ventricular myocytes[J]. Br J Pharmacol, 2007, 150(6): 720-6. doi: 10.1038/sj.bjp.0707147 [9] Okazaki R, Iwasaki YK, Miyauchi Y, et al. Lipopolysaccharide induces atrial arrhythmogenesis via down-regulation of l-type Ca2 + Channel genes in rats[J]. Int Heart J, 2009, 50(3): 353-63. doi: 10.1536/ihj.50.353 -
下载:
点击查看大图
图(2)
计量
- 文章访问数: 515
- HTML全文浏览量: 218
- PDF下载量: 3
- 被引次数: 0
