Manual preparation method and applications of paraffin-embedded cell block and cell microarray
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摘要:
目的介绍一种石蜡包埋细胞块及细胞芯片的简易制作方法及验证其应用价值。 方法收集临床送检细胞学标本，采用简易方法制作成石蜡包埋的细胞块及细胞芯片，HE染色、免疫细胞化学染色及TUNEL检测法验证其应用价值。 结果此方法能简易制作出细胞块及60个点阵左右的细胞芯片，经HE染色、免疫组化染色及TUNEL凋亡染色证实，染色效果良好，结果可靠。 结论本方法制作的石蜡包埋细胞块及细胞芯片的操作容易，设备简单，细胞形态和抗原保持良好，值得推广应用。 Abstract:ObjectiveTo introduces a simple preparation method of paraffin-embedded cell block and cell microarray and verify its application value. MethodsCollecting clinical cytology specimens and then making paraffin embedding cell block and cell microarray by manual and verify its application value by HE staining, immunocytochemistry staining and TUNEL technique. ResultsThis method can produced cell block and about 60 lattice cell microarray. The dyeing effect of cell block and cell microarray are reliable confirmed by HE staining, immunocytochemistry staining and TUNEL technique. ConclusionAs the manual preparation method of paraffin-embedded cell block and microarray required are simple and easy to operate, the method can be maintained well cellular morphology and antigenicity and worth popularizing and applying. -
Key words:
- cell block /
- cell microarray /
- paraffin-embedded /
- immunocytochemistry /
- TUNEL
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