Abdominal MRI studies of SD rat breathe anesthetic of dose control
-
摘要:
目的探讨在7.0T小动物磁共振研究中,呼吸麻醉剂量对实验动物和图像质量的影响及临床意义。 方法选取80只130 g左右的SD大鼠,采用2.0 lps氧气加0.5%异氟烷,进行诱导麻醉,待麻醉完全后,移至检查床,按持续麻醉剂量进行分组,分为0.1%,0.15 %,0.2%,0.25%四组,每组20只,进行大鼠腹部T2 WI成像。 结果80例实验动物共死亡4只,实验中进行二次麻醉15只,完成实验76只,与A、B、D组对比,C组的安全性及实用性最好,其差异有统计学意义。 结论小动物腹部实验中,实验动物的呼吸质量直接影响图像的质量,实验的进程及实验结果,对病变的诊断有着直接的影响,适量的麻醉能有效控制实验动物的状态,提高实验的质量,为临床的诊断提供实验保障。 Abstract:ObjectiveTo discuss the effects of breathe anesthesia on experimental and clinical significance in 7.0 T animals magnetic resonance (NMR) studies. MethodsSelected 80 SD rats, each 130 g, used oxygen (2.0 lps) plus isoflurane (0.5%) for the induction of anesthesia. After anesthesia completely, moved the rats to check bed, then devided the rats into 4 groups according to the continuous anesthesia dose (0.1%, 0.15%, 0.2%, 0.25%), with 20 rats in each group, for T2 WI imaging in rat's abdomen. ResultsIn the experiments, 4 rats were death, 15 used a second anesthesia and 76 rats completed the experiments, compared with A、B、D groups, group C was best in the security and practicability, the difference was statistically significant. ConclusionIn the experiments, the quality of breathing can directly affect the quality of the images, the experiment process and results have a direct impact to the diagnosis of lesions. Proper anesthesia dose can effectively control the status of the experimental animals, improve the quality of the experiment and provide experiment protection for clinical diagnosis. -
Key words:
- MRI /
- anesthesia breathing /
- imaging systems
-
表 1 四组大鼠检查结果
Table 1. The results of examine in four groups of rats
Group Deaths Numbers of secondary anesthesia The average inspection time Image quality A 0 10 16 min 40 s + B 0 5 12 min 5 s ++ C 0 0 7 min 10 s +++ D 4 0 6 min 30 s +++ Note: + On behalf of image clarity. -
[1] 高天舒, 邹飞燕, 唐运莲, 等.速眠新复合麻醉对外科手术实验用犬的效果观察[J].中国比较医学杂志, 2006, 16(6): 339-40. http://www.cnki.com.cn/Article/CJFDTOTAL-ZGDX200606007.htm [2] 梁佩红, 江斌, 陈居铕, 等.三种麻醉方法在骨科动物实验中效果比较[J].中国比较医学杂志, 2012, 22(11): 59-62. http://www.cnki.com.cn/Article/CJFDTOTAL-ZGDX201211014.htm [3] 张安君, 赵喜, 王国年, 等.小孔径正交相控阵线圈与临床表面柔性线圈小动物MRI成像质量的比较研究[J].中国中西医结合影像学杂志, 2010, 8(3): 196-8. http://www.cnki.com.cn/Article/CJFDTOTAL-JHYX201003005.htm [4] 谢萍, 王喆, 陆承荣, 等.氯胺酮与速眠新、硫喷妥钠对犬麻醉效果的观察[J].中国比较医学杂志, 2008, 18(4): 64-5. http://www.cnki.com.cn/Article/CJFDTOTAL-ZGDX200804021.htm [5] 章根木.实验动物气体吸入型麻醉技术[J].实验动物科学, 2007, 24 (4): 42-9. http://www.cnki.com.cn/Article/CJFDTOTAL-SYDG200704015.htm [6] 谭永红, 徐世元, 范凤飞.大鼠脊髓针刺损伤程度与脊髓功能变化的相关性研究[J].南方医科大学学报, 2012, 32(3): 333-5. http://www.cnki.com.cn/Article/CJFDTOTAL-DYJD201203009.htm [7] 张洁, 王勇, 韩雪萍.输尿管镜手术中不同剂量盐酸戊乙奎醚应用105例体会[J].郑州大学学报:医学版, 2009, 1: 200-2. http://www.cnki.com.cn/Article/CJFDTOTAL-HNYK200901070.htm [8] 张学康, 李梦圆.术中持续输注小剂量艾司洛尔对瑞芬太尼麻醉患者心血管反应的影响[J].实用临床医药杂志, 2014, 18(3): 39-40. http://www.cnki.com.cn/Article/CJFDTOTAL-XYZL201403012.htm [9] 李萌萌, 张庆红, 刘瑛辉, 等.手术室外精神发育迟滞患者外周血单个核细胞移植术的麻醉管理[J].南方医科大学学报, 2011, 31(7): 1193-5. http://www.cnki.com.cn/Article/CJFDTOTAL-DYJD201107022.htm