The distribution and characteristics of HBV genotypes between Hui and Han population in XinJiang
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摘要:
目的 了解新疆地区回族、汉族慢性乙型肝炎患者 HBV 基因型分布特点,完善新疆少数民族地区 HBV 流行病学资料。 方法对来自乌鲁木齐和伊犁地区的回族与汉族乙型肝炎患者共 194 例,收集血清样本,应用 ELISA 法检测乙肝五项,测序方法进行 HBV 基因分型,荧光定量 PCR 法检测 HBV 基因载量。 结果 94 例回族患者中各基因型比例分别为:B 型 7 例占 7.44%,C 型 66 例占 70.21%,D 型 21 例占 22.34%。100 例汉族患者中各基因型比例分别为:B 型 23 例占 23%,C 型 57 例占 57%,D 型 12 例占12%,B/C 混合型 8 例占 8%。汉族和回族中均以 C 型占优势,但 4 种基因型在汉族和回族中的总体分布不同:C 型和 D 型在回族的分布频率大于汉族(P=0.004 和 P=0.002),而 B 型和混合型在汉族中的分布频率大于回族(P=0.003 和 P=0.007)。HBV 基因载量测定检测结果显示,回、汉两民族间 D 基因型在 HBV-DNA 拷贝数上有显著差异(P<0.05),其他型别均无统计学意义。 结论 HBV 的 4 种基因型在汉族和回族中总体分布不同,两民族 C 型都占有绝对优势,但基因类型构成又各具特点,体现了新疆地区 HBV 基因型的地域分布和族群分布特点。 Abstract:Objective To study the distribution characteristics of HBV genotypes in Hui,Han patients with chronic hepatitis B virus (HBV) and epidemiological data of HBV in Xinjiang. Methods The five serological markers were detected by ELISA,HBV genotyping was performed by genome sequencing method,HBV load were detected by fluorogenic quantitative PCR. Results Type B in 7 cases (7.44%)、type C in 66 cases (70.21%) and type D in 21 cases (22.34%) were genotyped in 94 Hui cases. Type B in 23 cases (23%),type C in 57 cases (57%),type D in 12 cases (12%) and 8 cases of BC type (8%) were genotyped in 100 Han cases. The results showed that HBV genotype in two people were mostly of type C,type D ratio in Hui population was significantly higher than that in Han population. HBV gene load tests showed that there were statistically significant difference among genotype D in Hui patients. Conclusion In Xinjiang the main HBV genot ype were C and D type in Hui patients while B and C type were main genotyoe in Han patients. Genotype C has an absolute advantage in both Hui and Han patients. But composition of genotypein two population had its own characteristics. It showed the ethnic and regional distribution characteristic of HBV genotype in Xinjiang minority area. -
Key words:
- Hepatitis B virus /
- Genotype /
- Hui /
- Han population /
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表 1 回族患者HBV基因型分布及HBV DNA拷贝数比较
HBV基因型 回族 汉族 P 例数(%) HBV-DNA 载量* 例数(%) HBV-DNA 载量* 基因型比例 HBV-DNA 载量 B 7(7.45) 4.95±0.398 23(23.00) 5.32±0.231 0.003 >0.05 C 66(70.21) 4.37±0.523 57(57.00) 5.06±0.174 0.004 >0.05 D 21(22.34) 3.09±0.194 12(12.00) 6.01±0.269 0.002 <0.001 B/C 0 NA 8(8.00) 4.69±0.065 0.007 >0.05 注:*HBV DNA拷贝数为lgcopies/ml,NA:无获得数据。 表 2 不同性别、年龄回族与汉族患者HBV基因型分布比较(n,×10-2)
对比项目 例数 B型 C 型 D 型 混合型 回 汉 回 汉 回 汉 回 汉 回 汉 性别 男 50 63 5(71) 14(61) 37(56) 36(63) 8(38) 8(67) 0 5(63) 女 44 37 2(29) 9(39) 29(44) 21(37) 13(62) 4(33) 0 3(37) 年龄(岁) ≤30 29 43 3(43) 10(43) 16(24) 24(42) 10(48) 4(34) 0 5(63) 30~50 40 53 3(43) 12(53) 29(44) 31(54) 8(38) 7(58) 0 3(37) ≥50 25 4 1(14) 1(4) 21(32) 2(4) 3(14) 1(8) 0 0 注:≤30和≥50比较χ2=17.528,P<0.001;30~50岁和50岁比较χ2=16.571,P<0.001。 表 3 不同类型HBV感染者中HBV基因型分布
临床类型 例数 B型 C型 D型 BC混合型 回族 汉族 回族 汉族 回族 汉族 回族 汉族 无症状携带者 19 1 (5.3) 1( 5.3) 7(36.8) 1(5.3) 8(42.1) 1(5.3) 0 0 急性乙型肝炎 13 1 (7.7) 2(15.4) 3(23.1) 5(38.5) 1 (7.7) 1(7.7) 0 0 慢性乙型肝炎 138 5 (3.6) 16(11.6) 48(34.9) 42(30.4) 11( 8.0) 9(6.5) 0 7(5.1) 肝硬化 16 0 2(12.5) 5(31.3) 6(37.5) 1( 6.3) 1(6.3) 0 1(6.3) 肝细胞癌 8 0 2(25.0) 3(37.5) 3(37.5) 0 0 0 0 合计 194 -
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