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股骨头和血清LncRNA HOTAIR表达与非创伤性股骨头坏死进展的相关性

刘伟乐 郑少伟 黎旭 王胤 孙春汉 谢福杰 钟浩博

刘伟乐, 郑少伟, 黎旭, 王胤, 孙春汉, 谢福杰, 钟浩博. 股骨头和血清LncRNA HOTAIR表达与非创伤性股骨头坏死进展的相关性[J]. 分子影像学杂志, 2021, 44(3): 482-487. doi: 10.12122/j.issn.1674-4500.2021.03.13
引用本文: 刘伟乐, 郑少伟, 黎旭, 王胤, 孙春汉, 谢福杰, 钟浩博. 股骨头和血清LncRNA HOTAIR表达与非创伤性股骨头坏死进展的相关性[J]. 分子影像学杂志, 2021, 44(3): 482-487. doi: 10.12122/j.issn.1674-4500.2021.03.13
Weile LIU, Shaowei ZHENG, Xu LI, Yin WANG, Chunhan SUN, Fujie XIE, Haobo ZHONG. Correlation of local and serum LncRNA HOTAIR expressions with disease severity of non-traumatic ONFH[J]. Journal of Molecular Imaging, 2021, 44(3): 482-487. doi: 10.12122/j.issn.1674-4500.2021.03.13
Citation: Weile LIU, Shaowei ZHENG, Xu LI, Yin WANG, Chunhan SUN, Fujie XIE, Haobo ZHONG. Correlation of local and serum LncRNA HOTAIR expressions with disease severity of non-traumatic ONFH[J]. Journal of Molecular Imaging, 2021, 44(3): 482-487. doi: 10.12122/j.issn.1674-4500.2021.03.13

股骨头和血清LncRNA HOTAIR表达与非创伤性股骨头坏死进展的相关性

doi: 10.12122/j.issn.1674-4500.2021.03.13
基金项目: 

惠州市临床重点专科培育项目 惠市卫函[2019]462

惠州市临床重点专科培育项目 惠市卫函[2018]716

广东省医学科研基金 B2012373

惠州市科技计划 2020Y049

广东省中医药局科研项目 20202215

详细信息
    作者简介:

    刘伟乐,副主任医师,E-mail: zswljq@126.com

    通讯作者:

    钟浩博,副主任医师,E-mail: 522948721@qq.com

Correlation of local and serum LncRNA HOTAIR expressions with disease severity of non-traumatic ONFH

  • 摘要: 目的探讨非创伤性股骨头坏死(ONFH)患者血清和股骨头局部LncRNA HOX转录反义RNA(HOTAIR)的表达与疾病严重程度的关系。方法纳入本院接收的保髋或全髋置换的非创伤性ONFH患者90例,健康对照84例。RT-PCR检测血清和局部HOTAIR的表达,影像学进展由国际骨循环研究学会(ARCO)分期判定,采用VAS和Harris髋关节评分评价ONFH患者临床严重程度,应用ROC曲线确定血清HOTAIR在影像学进展中的诊断价值。结果非创伤性ONFH患者血清HOTAIR表达高于健康对照组(1.97±0.32 vs 1.00±0.10,P < 0.001),ONFH组织中的局部HOTAIR在坏死区高于非坏死区(2.37±0.25 vs 1.00±0.10,P < 0.001)。ARCO 4级的ONFH患者血清和局部HOTAIR表达高于3级(2.22±0.28 vs 1.94±0.36,P=0.001;2.58±0.22 vs 2.42±0.32,P=0.02)。ARCO 3级的ONFH患者与ARCO 1/2级相比,血清HOTAIR表达升高(1.94±0.36 vs 1.73±0.23,P=0.009;2.42±0.32 vs 2.13±0.24,P < 0.001)。血清中和局部HOTAIR的表达与ARCO分级呈显著正相关(r=0.569,P < 0.001;r=0.585,P < 0.001)。血清和局部HOTAIR表达与VAS评分呈正相关(血清r=0.557,P < 0.001;局部r=0.672,P < 0.001),与HSS评分呈显著负相关(血清r=-0.326,P=0.002;局部r=-0.489,P < 0.001)。ROC曲线分析显示,血清HOTAIR可作为诊断非创伤性ONFH影像学进展的良好指标(AUC=0.663, P=0.030; AUC=0.726, P=0.003)。结论非创伤性ONFH患者血清和局部HOTAIR表达升高可能反映ONFH病情的严重程度。

     

  • 图  1  ARCO分期1~4期X线股骨头坏死改变

    Figure  1.  X-ray changes of ONFH in ARCO stage 1-4.

    图  2  HOTAIR在ONFH患者中的表达水平

    A:激素型ONFH和酒精型ONFH血清HOTAIR相对表达水平;B:ONFH患者与对照组中血清HOTAIR相对表达水平;C:ONFH和对照组HOTAIR相对表达水平;**P<0.01.

    Figure  2.  HOTAIR expressions in ONFH patients.

    图  3  血清与局部HOTAIR在不同ARCO分期时期的表达程度

    A:血清HOTAIR在不同ARCO分期中的表达水平;B:局部HOTAIR在不同ARCO分期中的表达水平;C:血清HOTAIR与ARCO分期的相关性;D:局部HOTAIR与ARCO分期的相关性

    Figure  3.  Serum and local HOTAIR expressions among different ACRO stages.

    图  4  ONFH患者血清与局部HOTAIR表达与临床症状严重的相关性

    A:血清HOTAIR表达与VAS评分的相关性;B:局部HOTAIR表达与VAS评分的相关性;C:血清HOTAIR表达与HHS评分的相关性;D:局部HOTAIR表达与VAS评分的相关性.

    Figure  4.  Correlation of serum and local HOTAIR expressions with clinical severity in ONFH patients.

    图  5  血清HOTAIR水平的ROC曲线分析

    A:血清HOTAIR水平ARCO 1/2 vs ARCO 3分期的ROC曲线分析;B:血清HOTAIR水平ARCO 3 vs ARCO 4分期的ROC曲线分析.

    Figure  5.  A ROC curve analysis of HOTAIR expressions.

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出版历程
  • 收稿日期:  2021-03-06
  • 刊出日期:  2021-05-20

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