Application of stochastic optical reconstruction microscopy in the observation of exosomes

Di MA; Renpin XIA; Zhijian FENG; Xiaoyan YANG; Juelan FU; Gefu MA

doi:10.3969/j.issn.1674-4500.2018.02.20

Volume 41 Issue 2

May 2018

Turn off MathJax

Article Contents

Article Navigation > Journal of Molecular Imaging > 2018 > 41(2): 224-228

Di MA, Renpin XIA, Zhijian FENG, Xiaoyan YANG, Juelan FU, Gefu MA. Application of stochastic optical reconstruction microscopy in the observation of exosomes[J]. Journal of Molecular Imaging, 2018, 41(2): 224-228. doi: 10.3969/j.issn.1674-4500.2018.02.20

Citation:

Di MA, Renpin XIA, Zhijian FENG, Xiaoyan YANG, Juelan FU, Gefu MA. Application of stochastic optical reconstruction microscopy in the observation of exosomes[J]. Journal of Molecular Imaging, 2018, 41(2): 224-228. doi: 10.3969/j.issn.1674-4500.2018.02.20

Citation:

PDF( 2123 KB)

Application of stochastic optical reconstruction microscopy in the observation of exosomes

doi: 10.3969/j.issn.1674-4500.2018.02.20

1.
Department of Kidney Medicine, The Second Afiliated Hospital of Kunming Medical University, Kunming 650500,China
2.
Department of Transplantation, The First Afiliated Hospital of Kunming Medical University, Kunming 650032,China
3.
Clinical Laboratory,Tianqi Hospital,The Second Afiliated Hospital of Kunming Medical University,Kunming 650500,China

Received Date: 2018-02-19
Publish Date: 2018-04-01

Abstract

Abstract

Objective To investigate the application value of stochastic optical reconstruction microscopy (STORM) in the observation of exosomes. Methods In the experiment, exosomes were extracted by adding EXOQUICK-TC to the culture media supernatants of primarily cultured parathyroid cells from patients with hyperparathyroidism. After the immunofluorescence labeling of the specific transmembrane protein CD63 on the surface of exosomes, STORM was applied to perform ultra-high resolution imaging and diameter measurement of exosomes. Results In the experiment, STORM successfully performed the single molecule positioning, diameter measurement and ultra-high resolution imaging of exosomes extracted from the culture media supernatants of parathyroid cells of patients with hyperparathyroidism. Conclusion Compared to conventional optical microscopy, STORM, with its unique optical characteristics which has broken through the optical diffraction limit, has a resolution of 20-50 nm and can perform single-molecular acute positioning, diameter measurement and ultra-high resolution imaging of exosomes. In view of these outstanding features, we believe that STORM and other ultra-high resolution imaging technologies will play vital roles in the subsequent research on exosomes and exosome-related physiological procedures.
- exosome,
- stochastic optical reconstruction microscopy,
- super resolution imaging

FullText(HTML)

References(23)

References

[1]	Liu W, Ridefelt P, Akerström G, et al. Differentiation of human parathyroid cells in culture[J]. J Endocrinol, 2001, 168(3): 417-25. doi: 10.1677/joe.0.1680417
[2]	王宁宁, 王笑云, 陆福明, 等. 肾性甲旁亢患者原代培养的甲状旁腺细胞PTH分泌的变化[J]. 肾脏病与透析肾移植杂志, 2000, 9(02): 193-5. doi: 10.3969/j.issn.1006-298X.2000.02.025
[3]	Goodman WG, Quarles LD. Development and progression of secondary hyperparathyroidism in chronic kidney disease: lessons from molecular genetics[J]. Kidney Int, 2008, 74(3): 276-88. doi: 10.1038/sj.ki.5002287
[4]	Zlotogorski-Hurvitz A, Dayan D, Chaushu G, et al. Human saliva-derived exosomes: comparing methods of isolation[J]. J Histochem Cytochem, 2015, 63(3): 181-9. doi: 10.1369/0022155414564219
[5]	Pan BT, Johnstone RM. Fate of the transferrin receptor during maturation of sheep reticulocytes in vitro: selective externalization of the receptor[J]. Cell, 1983, 33(3): 967-78. doi: 10.1016/0092-8674(83)90040-5
[6]	Gholizadeh S, Shehata Draz M, Zarghooni M, et al. Microfluidic approaches for isolation, detection, and characterization of extracellular vesicles: Current status and future directions[J]. Biosens Bioelectron, 2017, 91(2): 588-605. https://www.sciencedirect.com/science/article/pii/S0956566316313124
[7]	Théry C, Ostrowski M, Segura E. Membrane vesicles as conveyors of immune responses[J]. Nat Rev Immunol, 2009, 9(8): 581-93. doi: 10.1038/nri2567
[8]	Akers JC, Gonda D, Kim R, et al. Biogenesis of extracellular vesicles (EV): exosomes, microvesicles, retrovirus-like vesicles, and apoptotic bodies[J]. J Neurooncol, 2013, 113(1): 1-11. doi: 10.1007/s11060-013-1084-8
[9]	Vlassov AV, Magdaleno S, Setterquist R, et al. Exosomes: current knowledge of their composition, biological functions, and diagnostic and therapeutic potentials[J]. Biochim Biophys Acta, 2012, 1820(7): 940-8. doi: 10.1016/j.bbagen.2012.03.017
[10]	Pant S, Hilton H, Burczynski ME. The multifaceted exosome: biogenesis, role in normal and aberrant cellular function, and frontiers for pharmacological and biomarker opportunities[J]. Biochem Pharmacol, 2012, 83(11): 1484-94. doi: 10.1016/j.bcp.2011.12.037
[11]	Zhan R, Leng X, Liu X, et al. Heat shock protein 70 is secreted from endothelial cells by a non-classical pathway involving exosomes[J]. Biochem Biophys Res Commun, 2009, 387(2): 229-33. doi: 10.1016/j.bbrc.2009.06.095
[12]	Coppieters K, Barral AM, Juedes A, et al. No significant CTL cross-priming by dendritic cell-derived exosomes during murine lymphocytic choriomeningitis virus infection[J]. J Immunol, 2009, 182(4): 2213-20. doi: 10.4049/jimmunol.0802578
[13]	Théry C, Duban L, Segura E, et al. Indirect activation of naïve CD4+ T cells by dendritic cell-derived exosomes[J]. Nat Immunol, 2002, 3(12): 1156-62. doi: 10.1038/ni854
[14]	Villarroya-Beltri C, Gutiérrez-Vázquez C, Sánchez-Madrid F, et al. Analysis of microRNA and protein transfer by exosomes during an immune synapse[J]. Methods Mol Biol, 2013, 1024(1024): 41-51. doi: 10.1007/978-1-62703-453-1_4
[15]	Wen D, Peng Y, Liu D, et al. Mesenchymal stem cell and derived exosome as small RNA carrier and Immunomodulator to improve islet transplantation[J]. J Control Release, 2016, 238(10): 166-75. https://www.sciencedirect.com/science/article/pii/S0168365916304904
[16]	Ostenfeld MS, Jeppesen DK, Laurberg JR, et al. Cellular disposal of miR23b by RAB27-dependent exosome release is linked to acquisition of metastatic properties[J]. Cancer Res, 2014, 74(20): 5758-71. doi: 10.1158/0008-5472.CAN-13-3512
[17]	Franzen CA, Blackwell RH, Todorovic V, et al. Urothelial cells undergo epithelial-to-mesenchymal transition after exposure to muscle invasive bladder cancer exosomes[J]. Oncogenesis, 2015, 4(11): e163-8.
[18]	Jeppesen DK, Nawrocki A, Jensen SG, et al. Quantitative proteomics of fractionated membrane and lumen exosome proteins from isogenic metastatic and nonmetastatic bladder Cancer cells reveal differential expression of EMT factors[J]. Proteomics, 2014, 14(6): 699-712. doi: 10.1002/pmic.v14.6
[19]	Bates M, Jones SA, Zhuang X. Stochastic optical Reconstruction microscopy (STORM): a method for superresolution fluorescence imaging[J]. Cold Spring Harb Protoc, 2013,13(6): 498-520.
[20]	Rust MJ, Bates M, Zhuang X. Sub-diffraction-limit imaging by stochastic optical Reconstruction microscopy (STORM)[J]. Nat Methods, 2006, 3(10): 793-5. doi: 10.1038/nmeth929
[21]	杨洁, 田翠萍, 钟桂生. 随机光学重构显微成像技术及其应用[J]. 光学学报, 2017,16(03): 51-63. http://mall.cnki.net/magazine/Article/GXXB201703006.htm
[22]	Endesfelder U, Heilemann M. Direct stochastic optical Reconstruction microscopy (dSTORM)[J]. Methods Mol Biol, 2015, 1251(1): 263-76. doi: 10.1007/978-1-4939-2080-8_14
[23]	Chen C, Zong S, Wang Z, et al. Imaging and intracellular tracking of cancer-derived exosomes using Single-Molecule Localization-Based Super-Resolution microscope[J]. Acs Appl Mater Interfaces, 2016, 8(39): 25825-33. doi: 10.1021/acsami.6b09442

Relative Articles

Supplements(0)

Cited By

Proportional views

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Figures(4)

Get Citation

PDF

XML

Article Metrics

Article views (1612) PDF downloads(4)

Application of stochastic optical reconstruction microscopy in the observation of exosomes

doi: 10.3969/j.issn.1674-4500.2018.02.20

Abstract

References

Proportional views

Catalog

通讯作者: 陈斌, bchen63@163.com

Article Metrics

Proportional views

Related

Application of stochastic optical reconstruction microscopy in the observation of exosomes

doi: 10.3969/j.issn.1674-4500.2018.02.20

Abstract

References

Proportional views

Catalog

通讯作者: 陈斌, bchen63@163.com

Article Metrics

Proportional views

Related

Export File

Citation

Format

Content