Preparation of aescinate and its effects on tissue edema rats model
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摘要:
目的 建立高纯度药用级别七叶皂苷的提取工艺并探讨其对组织水肿的治疗作用。 方法 利用醇提方法从梭罗果中获得七叶皂苷,随后使用软性填料的离子交换柱层析获得高纯度的七叶皂苷单体;使用单体制成喷雾剂对砸伤所致的组织水肿大鼠模型进行治疗。 结果 从梭罗果中利用醇提获得粗纯的七叶皂苷提取液,活性炭脱色并浓缩后利用离子交换柱层析纯化获得纯度>90%的七叶皂苷单体。以该单体为主要成分制成的喷雾剂对于砸伤所致大鼠组织水肿模型具有良好的治疗效果,显著减轻了后肢的水肿程度(P<0.05),病灶变小,炎性细胞和纤维细胞浸润减少,其效果等同或优于阳性对照药物云南白药。 结论 传统的用于蛋白质纯化的软琼脂糖凝胶在中药单体的提纯中可发挥重要作用;七叶皂苷钠对于砸伤所致组织水肿有着良好的疗效。 Abstract:Objective To prepare high purity pharmaceutically acceptable sodium aescinate from Chinese buckeye seed and investigate its therapeutic effect on tissue edema rats. Methods Sodium aescinate was firstly roughly extracted from Chinese buckeye seeds by ethanol and decolorized with activated carbon. Then, the extraction products were further purified by ion-exchange chromatography by loading onto a column packed with ion-exchanger medium. The final sodium aescinate products were used as spraying agent to treat tissue edema rat model. Results The purity of sodium aescinate was over 90% after extracted by ethanol and purified via ion-exchange chromatography. When used as spraying agent, sodium aescinate significantly attenuated the degree of edema in the hind limbs of rats, the lesions became smaller, the infiltration of inflammatory cells and fibroblasts was apparently reduced, and the effect was equal or even better than that of the positive control Yunnan Baiyao. Conclusion Soft sepharose beads which are traditionally used for protein purification can be effectively used for Traditional Chinese medicine monomer preparation. Sodium aescinate may be a potential agent for tissue edema treatment. -
Key words:
- sodium aescinate /
- Chinese buckeye seed /
- tissue edema /
- ion-exchange chromatography
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表 1 七叶皂苷喷雾剂配方
序号 计量区分 原料名 配方量(%) 用途 备注 1 C 0.47%七叶皂苷水溶液 43.45 基料 2 B 95%乙醇 19.00 基料 也可是溶解剂 3 D 氨基酸保湿剂(NMF-50) 4.00 功能料 4 D 丙二醇 2.00 助溶剂 保湿和润滑剂 5 D 甘油 2.00 助溶剂 保湿和润滑剂 6 A 红没药醇 0.80 功能料 消炎 7 D 水溶性维生素E(Ve) 0.40 功能料 改善微循环 8 A 玉洁新 0.20 功能料 杀菌剂 9 E 液体防腐剂(GMP-1) 0.15 防腐剂 10 F 盐酸利多卡因 2.00 局麻剂 -
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