The ExPression and clinical significance of eIF4E and ki67 in patients with endometrosis
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摘要:
目的探讨eIF4E和ki67在子宫内膜异位症中的表达及临床意义。 方法采用免疫组化技术SP法检测异位内膜组(30例),在位内膜组(30例)及正常内膜组(20例)中eIF4E及ki67蛋白的表达水平。 结果eIF4E和ki67在3组内膜中表达强弱依次为异位内膜>在位内膜>正常内膜,其差异有显著统计学意义(P<0.01);eIF4E在正常内膜组和在位内膜中分泌期高于增殖期,其差异有显著统计学意义(P<0.05),ki67在正常内膜组分泌期高于增殖期(P<0.05),在位内膜组增殖期高于分泌物(P<0.05);而eIF4E和ki67两者在异位内膜和在位内膜中的表达呈正相关(r=0.861,P<0.01;r=0.788,P<0.01),在正常内膜表达无明显相关性(r=0.297,P=0.207)。 结论eIF4E和ki67在子宫内膜异位症表达明显增高,eIF4E和ki67可能与子宫内膜异位症发生发展密切相关。 Abstract:Objective To explore the expression and clinical significance of eIF4E and ki67 in endometriosis. Methods The expression of eIF4E and ki67 were detected by immuno- histochemical method of SP in 30 cases of endometriosis and 20 cases of normal endometrium. Results Expression of the three groups showed such sequence as follows about eIF4E and ki67: ectopic endometrium>eutopic endometrium>normal endometrium, there was a significant difference statistically (P<0.01); eIF4E during the reign of normal endometrium and endometrial secretion period is higher than the proliferation period, the differences are significant statistical significance (P<0.05), ki67 in normal secretory phase endometrium group is higher than breeding period (P<0.05), in ectopic group multiplication period is higher than the discharge (P<0.05). The eIF4E and ki67 protein had obvious positive correlation in ectopic endometrial and endometrium (r=0.861, P<0.01; r=0.788, P<0.01), but there was no correlation in the normal endometrium (r=0.297, P=0.207). Conclusion eIF4E and ki67 in endometriosis express obviously increased. The expression of eIF4E and ki67 were positively associated with development and progression of endometriosis. -
Key words:
- Endometriosis /
- eIF4E /
- ki67 /
- immunohistochemical
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表 1 3组内膜中eIF4E蛋白的MOD值
Table 1. MOD value of eIF4E protein in three groups (Mean±SD)
组别 n MOD F P Z P 正常内膜组 20 0.102±0.008 -5.902① 0.000① 在位内膜组 30 0.144±0.016 64.93 0.000 -5.575② 0.000② 异位内膜组 30 0.232±0.029 -6.067③ 0.000③ ▲①对比异位内膜:Z=-5.902, P=0.000;②对比正常位内膜:Z=-5.575, P=0.000;③对比在位内膜:Z=-6.067, P=0.000. 表 2 3组内膜中ki67蛋白的MOD值
Table 2. MOD value of ki67 Protein in three grouPs (Mean±SD)
组别 n MOD F P Z P 正常内膜组 20 0.082±0.013 -5.753① 0.000① 在位内膜组 30 0.135±0.011 56.37 0.000 -3.348② 0.001② 异位内膜组 30 0.185±0.040 -4.963③ 0.000③ ▲①对比异位内膜:Z=-5.753, P=0.000;②对比正常位内膜:Z=-3.348, P=0.001; ③对比在位内膜:Z=-4.963, P=0.000. 表 3 eIF4E和ki67蛋白的MOD值与内膜分期的关系
Table 3. The MOD value of eIF4E and ki67 in different menstrual Phase (Mean±SD)
组别 分期 n eIF4E T P Ki67 T P 正常内膜组 增殖期 13 0.103±0.011 11.295 0.004 0.069±0.015 5.788 0.03 分泌期 7 0.134±0.012 0.098±0.022 在位内膜组 增殖期 18 0.148±0.013 6.987 0.013 0.142±0.023 5.369 0.028 分泌期 12 0.162±0.014 0.125±0.018 -
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