Application of Micro-CT in whole-body fat scanning imaging of mice
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摘要:
目的 探讨扫描电压和X射线滤光片对小鼠全身脂肪扫描图像质量和对小鼠辐射剂量的影响。 方法 选取C57BL/6小鼠6只，3只为正常对照小鼠，3只为高脂喂养的肥胖小鼠。应用Micro-CT对小鼠全身进行成像扫描，统计不同扫描条件下对小鼠的辐射剂量；使用Analyze12.0分析软件对小鼠皮下和内脏脂肪进行提取，然后评价扫描图像质量以及脂肪分布。 结果 正常小鼠和肥胖小鼠的扫描结果表明，在扫描电压为70 kV和Al 1.0 mm X射线滤光片扫描条件下，小鼠皮下脂肪和内脏脂肪边界清晰且无伪影，图像质量高，辐射剂量小。使用Analyze 12.0软件分离的小鼠皮下脂肪和内脏脂肪连续且平滑，脂肪显示完整。 结论 本研究为临床研究患者皮下脂肪和内脏脂肪提供了理论依据，对CT扫描电压和X射线滤光片的选择提供了数据参考。在降低扫描电压和保证图像质量的前提下，最大限度的降低患者受到的辐射剂量。 Abstract:Objective To investigate the effects of scanning voltage and X-ray filter on image quality and radiation dose of whole body fat scanning in mice. Methods Six C57BL/6 mice were selected, including three were normal control mice and three obese mice fed with high fat diet. Micro-CT was used to scan the whole body of mice, and the radiation dose under different scanning conditions was calculated. The subcutaneous and visceral fat were extracted by Analyze12.0 software, and the image quality and fat distribution were evaluated. Results The scanning results of normal mice and obese mice showed that under the scanning voltage of 70 kV and Al 1.0 mm X-ray filter, the boundary between mice subcutaneous fat and visceral fat was clear and without artifacts, with high image quality and low radiation dose. The subcutaneous fat and visceral fat of mice separated by analyze12.0 software were continuous and smooth with complete fat display. Conclusion This study provides a theoretical basis for the clinical study of subcutaneous fat and visceral fat in patients, and provides data reference for the selection of CT scan voltage and filter. Under the premise of reducing the scanning voltage and ensuring the image quality, the radiation dose to the patient was reduced to the maximum. -
Key words:
- micro-CT /
- tube voltage /
- filter /
- fat /
- image quality
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图 1 小鼠Micro-CT扫描成像三视图
Figure 1. Three views of mouse micro-CT scanning imaging. A: Normal mice were scanned with 90 kV tube voltage and Cu 0.06 mm+Al 0.5 mm filter; B: Image of fat extraction and separation.
图 2 小鼠Micro-CT扫描成像三视图
Figure 2. Three views of mouse micro-CT scanning imaging. A: Normal mice were scanned with 70 kV tube voltage and Cu 0.06 mm+Al 0.5 mm filter; B: Image of fat extraction and separation.
图 3 小鼠Micro-CT扫描成像三视图
Figure 3. Three views of mouse micro-CT scanning imaging. A: Normal mice were scanned with 70 kV tube voltage and Al 0.5 mm filter; B: Image of fat extraction and separation.
图 4 小鼠Micro-CT扫描成像三视图
Figure 4. Three views of mouse micro-CT scanning imaging. A: Normal mice were scanned with 70 kV tube voltage and Al 1.0 mm filter; B: Image of fat extraction and separation.
图 5 小鼠Micro-CT扫描成像三视图
Figure 5. Three views of mouse micro-CT scanning imaging. A: Scanning image of obese mice under 90 kV tube voltage and Cu 0.06 mm+Al 0.5 mm filter; B: Image of fat extraction and separation.
图 6 小鼠Micro-CT扫描成像三视图
Figure 6. Three views of mouse micro-CT scanning imaging. A: Scanning image of obese mice under 70 kV tube voltage and Cu 0.06 mm+Al 0.5 mm filter; B: Image of fat extraction and separation.
图 7 小鼠Micro-CT扫描成像三视图
Figure 7. Three views of mouse micro-CT scanning imaging. A: Scanning image of obese mice under 70 kV tube voltage and Al 0.5 mm filter; B: Image of fat extraction and separation.
图 8 小鼠Micro-CT扫描成像三视图
Figure 8. Three views of mouse micro-CT scanning imaging. A: Scanning image of obese mice under 70 kV tube voltage and Al 1.0 mm filter; B: Image of fat extraction and separation.
表 1 不同扫描条件下正常小鼠不同部位脂肪体积
Table 1. Fat volume of different parts of normal mice under different scanning conditions (Mean±SD, n=3)
Voltage (kV) X-ray filter Subcutaneous fat (mm3) Visceral fat (mm3) Bone volume (mm3) Radiation dose（mGy） 90 Cu 0.06 mm+Al 0.5 mm 1361.72±113.21 314.55±41.29 734.39±50.16 169 70 Cu 0.06 mm+Al 0.5 mm 930.79±96.25** 271.08±46.59** 765.78±43.74 148** 70 Al 0.5 mm 536.68±97.85 353.25±60.73 746.74±38.25 214 70 Al 1.0 mm 1374.66±86.86## 628.93±50.27## 730.37±32.49 164## 50 Cu 0.06 mm+Al 0.5 mm - - 752.11±21.32 45 50 Al 0.5 mm - - 776.97±28.56 114 **P < 0.01 vs 90 kV scanning voltage and Cu 0.06 mm+Al 0.5 mm X-ray filter scanning conditions; ##P < 0.01 vs 70 kV scanning voltage and Al 0.5 mm X-ray filter scanning conditions; -: The boundary between fat and other tissues is blurred, and the corresponding fat volume cannot be analyzed. 
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表 2 不同扫描条件下肥胖小鼠不同部位脂肪体积
Table 2. Fat volume in different parts of obese mice under different scanning conditions (Mean±SD, n=3)
Voltage (kV) X-ray filter Subcutaneous fat (mm3) Visceral fat (mm3) Bone volume (mm3) Radiation dose（mGy） 90 Cu 0.06 mm+Al 0.5 mm 4946.47±26.17 4443.88±46.28 633.96±41.83 169 70 Cu 0.06 mm+Al 0.5 mm 4855.15±53.76** 4367.69±53.27** 608.91±56.27 148** 70 Al 0.5 mm 4902.01±88.39 4916.38±90.20 645.77±37.19 214 70 Al 1.0 mm 6140.39±62.10## 5415.32±72.13## 655.84±40.87 164## 50 Cu 0.06 mm+Al 0.5 mm - - 659.23±21.32 45 50 Al 0.5 mm - - 671.82±28.56 114 **P < 0.01 vs 90 kV scanning voltage and Cu0.06 mm + Al 0.5 mm X-ray filter scanning conditions; ##P < 0.01 vs 70 kV scanning voltage and Al 0.5 mm X-ray filter scanning conditions; -: The boundary between fat and other tissues is blurred, and the corresponding fat volume cannot be analyzed.
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