Application of two-photon imaging on cell transplantation and therapy
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摘要: 双光子成像技术具有活体三维深层成像的能力，是重要的活体成像工具，在针对生物组织相关的活体、原位研究中应用广泛。通过移植细胞建立研究模型，可以在真实的细胞微环境中进行过程与机理研究。结合双光子成像技术，可以对移植细胞进行在体形态学鉴定与功能评价，避免体外培养模型带来的差异。双光子成像技术促进了疾病模型的研究，为疾病与治疗等病理生理过程的研究提供了重要的研究手段。细胞移植已经用于中枢神经系统、心肌、骨髓以及抗肿瘤药物等研究模型建立中，本文对双光子成像技术的技术原理、应用领域进行综述，并探讨了该技术的发展前景。双光子成像技术以其较大的成像深度、较高的成像质量等特点，满足了在体成像的需求，在以动物为研究对象的研究中发挥了重要的作用，使我们对活细胞生理、病理和药理领域的认识得到极大的发展。Abstract: Two-photon imaging technique realizes in vivo 3D deep-tissue imaging. It is an important tool of in vivo imaging and applied in research on in vivo and in situ biological tissue broadly. Research model established by cell transplantation helps study of process and mechanism in the real microenvironment. Using two-photon imaging, morphological observation and functional evaluation about donor cells is realized in living tissue, eliminating differences in vitro tissue culture models. Research of disease models are promoted by utilizing two-photon imaging. This technique is an important tool for research on disease and therapy pathphysiologic events. Cell transplants are used to establish research models of central nervous system, myocardium, bone marrow and anticancer drug. This article reviews the application of research on these regions with two-photon imaging technique, and discusses the development of this technique.
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Key words:
- two-photon imaging /
- cell transplantation /
- in vivo imaging
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图 1 人ESC移植到新生小鼠脑部2月后不同脑部区域的轴突双光子图像
A：纹状体；B：胼胝体；C：内囊；D：丘脑[29]
Figure 1. Axonal two-photon images of different brain regions of newborn mice after human ESC transplantation
图 2 完整心脏中心肌细胞移植后的双光子图像
A：灌注rhod-2的小鼠心脏图像，包含表达EGFP的移植心肌细胞，宿主心肌细胞由于绿色的EGFP和红色的rhod-2叠加，显示为黄色；B：对A图中白线区域线扫描结果[36]
Figure 2. Two-photon images of intact central cardiac muscle cells after transplantation
图 3 对颅骨不同深度下的二维双光子图像
白色为颅骨中胶原骨信号，红色为DiD标记的细胞，黄色为自发荧光的细胞，绿色为成骨细胞[41]
Figure 3. Two-dimensional two-photon images of the skull at different depths
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