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七叶皂苷凝胶对宫颈炎大鼠的治疗作用

薛晓畅 成俊宏 郭红彬 王东方 张喜烨 袁晓峰 李志兰 王增禄

薛晓畅, 成俊宏, 郭红彬, 王东方, 张喜烨, 袁晓峰, 李志兰, 王增禄. 七叶皂苷凝胶对宫颈炎大鼠的治疗作用[J]. 分子影像学杂志, 2019, 42(3): 364-368. doi: 10.12122/j.issn.1674-4500.2019.03.20
引用本文: 薛晓畅, 成俊宏, 郭红彬, 王东方, 张喜烨, 袁晓峰, 李志兰, 王增禄. 七叶皂苷凝胶对宫颈炎大鼠的治疗作用[J]. 分子影像学杂志, 2019, 42(3): 364-368. doi: 10.12122/j.issn.1674-4500.2019.03.20
Xiaochang XUE, Junhong CHENG, Hongbin GUO, Dongfang WANG, Xiye ZHANG, Xiaofeng YUAN, Zhilan LI, Zenglu WANG. Preparation of aescinate gel and its effects on cervicitis model in rats[J]. Journal of Molecular Imaging, 2019, 42(3): 364-368. doi: 10.12122/j.issn.1674-4500.2019.03.20
Citation: Xiaochang XUE, Junhong CHENG, Hongbin GUO, Dongfang WANG, Xiye ZHANG, Xiaofeng YUAN, Zhilan LI, Zenglu WANG. Preparation of aescinate gel and its effects on cervicitis model in rats[J]. Journal of Molecular Imaging, 2019, 42(3): 364-368. doi: 10.12122/j.issn.1674-4500.2019.03.20

七叶皂苷凝胶对宫颈炎大鼠的治疗作用

doi: 10.12122/j.issn.1674-4500.2019.03.20
详细信息
    作者简介:

    薛晓畅,博士,副教授,硕士生导师,E-mail: xue_xiaochang@yahoo.com

    通讯作者:

    王增禄,教授,E-mail: zengluwang2006@126.com

Preparation of aescinate gel and its effects on cervicitis model in rats

  • 摘要: 目的 探讨七叶皂苷凝胶剂对于宫颈炎模型大鼠的治疗作用。方法 利用醇提方法从梭罗果中分离纯化获得七叶皂苷并制备成凝胶剂;利用25%苯酚胶浆在SD大鼠中诱导宫颈炎模型,随后局部注入七叶皂苷凝胶进行治疗,观察模型大鼠阴道口红肿情况及分泌物量的变化,取宫颈组织石蜡包埋后切片,HE染色观察组织病理改变。结果 七叶皂苷凝胶剂可明显加快大鼠宫颈炎模型动物损伤修复,显著减轻大鼠阴道口红肿及分泌物。HE染色表明七叶皂苷凝胶剂处理后大鼠的损伤部位炎性细胞浸润明显减少。结论 七叶皂苷钠凝胶剂对于大鼠宫颈炎有着良好的疗效,有望用于宫颈炎的治疗。

     

  • 图  1  苯酚胶浆诱导宫颈炎模型大鼠阴道外观

    A: 正常组;B: 模型组.

    图  2  苯酚胶浆诱导宫颈炎模型阴道至宫颈组织形态变化

    A: 正常组;B: 模型组.

    图  3  七叶皂苷钠凝胶剂有效善宫颈炎模型大鼠的体质量减轻

    ***P<0.001, *P<0.05.

    图  4  七叶皂苷钠凝胶剂明显减轻宫颈炎大鼠的组织水肿

    *P<0.05.

    图  5  七叶皂苷钠凝胶剂可有效缓解宫颈炎大鼠阴道红肿和脓性分泌物

    A: 宫颈炎大鼠模型阴道红肿评分;B: 宫颈炎大鼠模型脓性分泌物评分.

    图  6  七叶皂苷钠凝胶剂可有效缓解宫颈炎大鼠阴道红肿和脓性分泌物

    A: 正常组;B: 模型组;C: 基础方治疗组;D: 七叶皂苷低剂量组;E: 七叶皂苷高剂量组. B图中黑箭示炎性细胞,蓝箭示上皮内炎性细胞,蓝箭头示坏死性上皮细胞. bar=50 μm.

    表  1  七叶皂苷凝胶剂配方

    序号 原料名 配方量(%)
    1 0.47%七叶皂苷水溶液 43.45
    2 卡波姆940 0.60
    3 丁二醇 5.00
    4 羟苯甲酯 0.20
    5 柠檬酸 0.08
    6 L-精氨酸 1.25
    7 苯氧乙醇 0.20
    8 余量
    下载: 导出CSV
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出版历程
  • 收稿日期:  2019-04-30
  • 刊出日期:  2019-07-01

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